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Catalog Number | orb1227994 |
---|---|
Category | Proteins |
Description | Interleukin 6 Recombinant Protein (HEK293 Expressed) |
Tested applications | WB |
Tag | Tag free |
Form/Appearance | Lyophilized |
Purity | > 98% as determined by SDS-PAGE. |
MW | 20.8 kDa |
Target | IL6 |
UniProt ID | P05231 |
Protein Sequence | Val 30 - Met 212 |
Source | HEK293 cells |
Biological Activity | Immobilized Human IL-6, Tag Free at 5ug/mL (100uL/well) can bind Biotinylated Human IL-6 R alpha, His Tag with a linear range of 4-125 ng/mL. |
NCBI | NP_000591 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | IL6, HGF, Interleukin-6, BSF2, HSF, IFNB2 Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
Human IL-6, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
98.00% | |
68.94 kDa (predicted). Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. |
98.00% | |
58.2 kDa (predicted). Due to glycosylation, the protein migrates to 90-116 kDa based on Tris-Bis PAGE result. |
98.00% | |
83.8 kDa (predicted). Due to glycosylation, the protein migrates to 110-135 kDa based on Tris-Bis PAGE result. |
98.00% | |
56.3 kDa (predicted). Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result. |
98.00% | |
69.09 kDa (predicted). Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result. |