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Catalog Number | orb1227996 |
---|---|
Category | Proteins |
Description | Interleukin 4 Recombinant Protein (HEK293 Expressed) |
Tested applications | WB |
Tag | Tag free |
Form/Appearance | Lyophilized |
Purity | > 90% as determined by SDS-PAGE. |
MW | 14.9 kDa |
Target | IL4 |
UniProt ID | P05112 |
Protein Sequence | His 25 - Ser 153 |
Source | HEK293 cells |
Biological Activity | The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50 5X106 Unit/mg. |
NCBI | AAH67514 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 20 mM Na2HPO4, 200 mM NaCl, pH 7.0 |
Alternative names | IL4, BCGF1, BSF1 Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
Human IL-4, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
> 90% as determined by SDS-PAGE | |
60-70 kDa |
98.00% | |
16.03 kDa (predicted). Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result. |
98.00% | |
17.8 kDa (predicted). Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result. |