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Catalog Number | orb1262444 |
---|---|
Category | Antibodies |
Description | INSIG1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This INSIG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the N-terminal region of human INSIG1. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 30 kDa |
Target | INSIG1 |
UniProt ID | O15503 |
NCBI | O15503 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Insulin-induced gene 1 protein, INSIG-1, INSIG1 Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of INSIG1 Antibody in Hela cell line lysates (35 ug/lane)
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
INSIG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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