INH6

SKU: orb1301005

Description

INH6 is a potent and selective small molecule inhibitor that targets the Hec1/Nek2 protein interaction. By disrupting this complex, it induces chromosome misalignment, making it a valuable research tool for studying mitosis, kinetochore function, and cancer mechanisms in both cellular and biochemical assays.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 1001753-24-7
• MW: 322.42
• Purity: 99.17% (May vary between batches)
• Formula: C₁₉H₁₈N₂OS
• SMILES: Cc1cc(C)c(-c2csc(NC(=O)c3ccccc3)n2)c(C)c1
• Target: MAPK,Apoptosis,Microtubule Associated
• Solubility: DMSO:60 mg/mL (186.09 mM);Ethanol:9 mg/mL (27.91 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+90% Corn Oil:2.5 mg/mL (7.75 mM)

Bioactivity

• Target IC50:
  Hec1:2.4 μM.|NEK2:2.4 μM
• In Vitro:
  INH6 effectively targets the Hec1/Nek2 complex for degradation, and shows potent cell killing activity with IC50 of 1.7, 2.1, 2.4, and 2.5 μM in MDA-MB231, MDA-MB468, HeLa and K562 cell lines. INH6 also triggers mitotic abnormalities in HeLa cells, and induces apoptosis.
• Cell Research:
  Standard XTT assays with a four-day drug treatment procedure are performed to measure the dose-dependent cytotoxicity of INH analogs in cultured cells. Triplicate sets are measured and compiled for final data presentation. The assay is performed by using a commercial kit following the instructions. In principle, cells are plated on 96-well dishes one day before the drug treatment, followed by drug treatment on day 2 and XTT assay on day 5 after drug addition. The absorption at 595 nm is measured with a plate reader and converted to cell survival percentages in comparison to mock treated groups.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, inhibit, Inhibitor, INH6, INH-6, INH 6, Microtubule Associated, MicrotubuleAssociated, MB231, MB468

Compound Identifiers

PubChem CID

7918451