Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag

SKU: orb3158042

Description

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

Key Properties

• Expression System: E. coli
• Target: Polymerase acidic protein
• Reactivity: Virus
• Tag: C-10×His
• Molecular Weight: 26.5 kDa
• Expression Region: Met 1 - Gly 209
• Purity: 90%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -70°C
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, 0.5 M Arginine, 50% Glycerol, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Polymerase acidic protein

UniProt

P31343

RefSeq:P31343