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Catalog Number | orb1710802 |
---|---|
Category | Proteins |
Description | Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. |
Target | Hemagglutinin 1/HA 1 (Influenza Virus) |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Virus |
Form/Appearance | Powder |
Buffer/Preservatives | 0.1 M Sodium citrate, pH 5.5 |
Purity | 90% |
UniProt ID | Q80KD9 |
MW | 40.9 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 40.9 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Expression Region | Asp 19 - Arg 338 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
Greater than 95.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
98.00% | |
37.6 kDa (predicted); 55 kDa (reducing condition, due to glycosylation) |
98.00% | |
37.8 kDa (predicted); 49.2 kDa (reducing conditions) |
98.00% | |
57.7 kDa (predicted); 67, 43 and 30 kDa (reducing conditions) |
98.00% | |
57.6 kDa (predicted); 43 and 27 kDa (reducing conditions) |