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IL-33 Recombinant Protein
Description
Research Area
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Activates mouse and human ST2-dependent NF-kappaB pathway. |
| Target | Il33 |
| Tag | His Tag |
| Protein Sequence | Mouse IL-33 (aa 109-266) is fused at the C-terminus to a His-tag. |
| Purity | >90% (SDS-PAGE). Endotoxin level is less than 1EU/ μg purified protein (LAL test; Lonza). |
Storage & Handling
−| Storage | Working aliquots are stable for up to 3 months when stored at -20°C. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.2um-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM NaCl. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Recombinant Mouse IL-33 Protein [orb2994601]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 17.6 KDa. Observed: 17-22 KDa, reducing conditions
500 μg, 1 mg, 10 μg, 50 μgRecombinant Human IL-33 Protein [orb2995234]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 18.1 KDa. Observed: 18 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Rhesus macaque IL-33 Protein [orb2994225]
Unconjugated
Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.3 KDa. Observed: 21 KDa, reducing conditions
1 mg, 10 μg, 50 μg, 500 μg

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Protocol Information
IL-33 Recombinant Protein (orb1230264)
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