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Catalog Number | orb1231303 |
---|---|
Category | Proteins |
Description | IL-23 R Recombinant Protein |
Tested applications | ELISA, WB |
Tag | Fc Tag |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 64.5 kDa |
Target | IL23R |
UniProt ID | Q5VWK5 |
Protein Sequence | Gly 24 - Gly 355 |
Source | HEK293 cells |
NCBI | NP_653302.2 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Alternative names | IL-23 R,IL-23 Receptor Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 64.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human IL-23 R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His, Avitag&Tag Free at 2 ug/ml (100 uL/well) on streptavidin precoated (0.2 ug/well) plate
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result. |
WB | |
> 95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. | |
66.8 kDa | |
HEK293 cells |
WB | |
> 90% as determined by SDS-PAGE. | |
39.9 kDa | |
HEK293 cells |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human IL-23 R Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gly24-Gly355) of Human IL-23 R fused with mFc His Tag at the C-terminal. |