Il1b Antibody

SKU: orb1272467

Description

Il1b Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rIL-1-beta (rat Interleukin-1-beta).
• Target: Il1b
• Purification: Anti-rIL-1-beta specific antibody was purified by affinity chromatography employing immobilized rIL-1-beta matrix.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-1 betaIL-1 beta

To detect Rat IL-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Rat IL-1-beta (orb1272466) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1-beta.

To detect Rat IL-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat IL-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 0.25 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 0.25 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

Il1b

UniProt

Q63264

RefSeq:Q63264