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Catalog Number | orb1229912 |
---|---|
Category | Proteins |
Description | IL-18 Recombinant Protein |
Tag | His Tag |
Form/Appearance | Lyophilized |
Purity | > 97% (SDS-PAGE). Endotoxin level is less than 1EU/ μg protein (LAL test; Lonza). |
Target | IL18 |
UniProt ID | Q9JI71 |
Protein Sequence | Human IL-18 (aa 37-193) is fused at the N-terminus to a His-tag. |
Source | E. coli |
NCBI | NP_001553 |
Storage | Stable for at least 1 year after receipt when stored at -20˚°C. Working aliquots are stable for up to 3 months when stored at -20˚°C. |
Buffer/Preservatives | Lyophilized from 0.2um-filtered solution in PBS. Reconstituted in sterile H2O not less than 100 μg/ml, which can then be further diluted in other aqueous solutions. |
Alternative names | Interleukin-18, Interferon-gamma-inducing Factor, Read more... |
Note | For research use only |
Application notes | This recombinant proteins is for research use only. |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 19 kDa based on Tris-Bis PAGE result. |
IF, IHC-Fr, IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.3 kDa after removal of the signal peptide.The apparent molecular mass of IL18-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Greater than 85% as determined by SDS-PAGE. | |
26.1 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
19.7 kDa | |
E.coli |