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IL-18 Recombinant Protein
Description
Research Area
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Target | IL18 |
| Tag | His Tag |
| Protein Sequence | Human IL-18 (aa 37-193) is fused at the N-terminus to a His-tag. |
| Purity | >97% (SDS-PAGE). Endotoxin level is less than 1EU/ μg protein (LAL test; Lonza). |
Storage & Handling
−| Storage | Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from 0.2um-filtered solution in PBS. Reconstituted in sterile H2O not less than 100 μg/ml, which can then be further diluted in other aqueous solutions. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human IL18 Protein, hFc Tag [orb1173723]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.3 kDa after removal of the signal peptide.The apparent molecular mass of IL18-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgRecombinant Mouse IL-18BPd Protein [orb3002275]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 45.1 KDa. Observed: 60-90 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Mouse IL-18 Protein [orb3002400]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 19.7 KDa. Observed: 20 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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Protocol Information
IL-18 Recombinant Protein (orb1229911)
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