You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1229915 |
---|---|
Category | Proteins |
Description | IL-12 Recombinant Protein |
Tag | Fc Tag |
Form/Appearance | Lyophilized |
Purity | > 98% (SDS-PAGE). Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza). |
Target | Il12a |
UniProt ID | Q6QLQ4 |
Protein Sequence | The mouse IL-12 complex composed of the p40 subunit (aa 1-335) and the p35 subunit (aa 23-215) is fused through a polypeptide linker to the Fc region of human IgG1. |
Source | CHO cells |
NCBI | NP_032377 |
Storage | Stable for at least 1 year after receipt when stored at -20˚°C. Working aliquots are stable for up to 3 months when stored at -20˚°C. |
Buffer/Preservatives | Lyophilized from 0.2um-filtered solution in PBS. Reconstitute at 100 μg/ml in sterile PBS. |
Alternative names | IL12-p70, CLMF, Cytotoxic lymphocyte maturation fa Read more... |
Note | For research use only |
Application notes | This recombinant proteins is for research use only. |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by SDS-PAGE and HPLC. | |
57.2 kDa. 75.0 kDa is the observed value of non-reducing gel,and the reducing glue is the size of p40 and p35 subunits, and 57.2 kDa is the theoretical value. | |
Baculovirus |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 50-60 (Mouse IL12 Beta) & 22 ( Human IL23 Alpha) kDa based on Tris-Bis PAGE result. |
Greater than 95% as determined by SDS-PAGE. | |
39.7 kDa & 27.2 kDa | |
Mammalian cell |
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
59.0 kDa | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by reduced SDS-PAGE. | |
83.5 kDa | |
HEK293 cells |