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Description
Research Area
Immunology & Inflammation
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | CHO cells |
|---|---|
| Biological Activity | 6,000 units/ug as determined by ELISA. Bioactivity demonstrated in cytokine synthesis inhibition assay, measuring inhibition of IL-6 production by PU5-1 cells. |
| Target | Il10 |
| Tag | Fc Tag |
| Purity | >98% (SDS-PAGE). Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza). |
| Protein Sequence | The extracellular domain of mouse IL-10 (aa 1-178) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a. |
Storage & Handling
−| Storage | Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from 0.2um-filtered solution in PBS. Reconstitute at 100 μg/ml in sterile PBS. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-10, Cytokine Sythesis Inhibitory Factor, CSIF
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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