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Catalog Number | orb1229831 |
---|---|
Category | Proteins |
Description | IL-1 alpha Recombinant Protein |
Tag | Tag Free |
Form/Appearance | Lyophilized |
Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
MW | 18 kD |
Target | Il1a |
UniProt ID | P01582 |
Protein Sequence | Ser115-Ser270 |
Source | E. coli |
Biological Activity | ED50 is less than 0.01 ng/ml as determined by the dose-dependent stimulation of mouse D10S cells.Specific Activity of 1.0 x 108 IU/mg. |
NCBI | P01582 |
Storage | Lyophilized protein should be stored at -20˚°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7˚°C for 2-7 days.Aliquots of reconstituted samples are stable at -20˚°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 50mM TrisHCl, 200mM NaCl, pH 8.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
Alternative names | Interleukin-1 Alpha, IL-1 Alpha, Il1a, IL-1F1, IL- Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for biological assays. For research use only. |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
18.0 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
18.1 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
17.9 kDa | |
E.Coli |
> 95% as determined by SDS-PAGE | |
25 kDa |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.2 kDa after removal of the signal peptide.The apparent molecular mass of IL1A-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |