iFSP1

SKU: orb1309551

Description

iFSP1 is a potent and selective FSP1 (AIFM2) inhibitor with an EC50 of 103 nM, functioning independently of glutathione. It sensitizes cancer cells to ferroptosis inducers like RSL3 and uniquely induces ferroptosis in GPX4-knockout, FSP1-overexpressing cells, making it a valuable tool for investigating ferroptosis pathways in vitro.

Research Area

Cell Biology

Key Properties

• CAS Number: 150651-39-1
• MW: 323.35
• Purity: 99.74%
• Formula: C₂₀H₁₃N₅
• SMILES: Cc1ccc(cc1)-c1c(C#N)c(N)n2c3ccccc3nc2c1C#N
• Target: Ferroptosis
• Solubility: DMSO:25 mg/mL (77.32 mM)

Bioactivity

• Target IC50:
  FSP1:103 nM (EC50)
• In Vitro:
  iFSP1 (0.001-1 μM; 24 hours) is less effective than genetic deletion of FSP1, and its treatment in the FSP1-knockout background does not enhance RSL3-induced ferroptosis. iFSP1 (3 μM; 24 hours) causes significant toxicity of RSL3 in a panel of genetically engineered (FSP1-knockout) human cancer cell lines. AIFM2, now renamed ferroptosis suppressor protein 1 (FSP1), is identified as a previously unrecognized anti-ferroptotic gene. iFSP1 (0.001-1 μM; 24 hours) inhibits the growth of Gpx4-knockout cells in a dose-dependent manner but does not affect wild type cells. Treatment with the ferroptosis inhibitor Lip-1 protects GPX4-knockout cells from iFSP1-induced ferroptosis.

Storage & Handling

• Storage: The compound is unstable in solution. Please use soon | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cancer, A549, cell, inhibit, NCl-H1437, Inhibitor, iFSP 1, iFSP1, iFSP-1, Ferroptosis, Gpx4-knockout, FSP1-knockout, FSP1, SW620, RSL3, U-373

Compound Identifiers

PubChem CID

699043