Ido1 Antibody

SKU: orb344470

Description

IDO antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IF, IHC, IP, WB
• Dilution Range: ELISA: 1:5000-1:50000, IHC: 1:100-1:500, IF: 1:50-1:100, IP: 10-100 µL, WB: 1:500-1:1500
• Reactivity: Human, Mouse
• Application Notes:
  Anti-IDO1 antibody has been tested in SDS-PAGE, ELISA, IP, and Western Blot. This antibody is suitable for use in IHC and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3
• Clone No.: 10.1
• Immunogen: Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.
• Target: Ido1
• Purity: Anti-IDO1 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human and mouse IDO1 protein. A BLAST analysis was used to suggest cross-reactivity with IDO1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IDO1 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-IDO1 antibody, Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido1, Ido-1

Western Blot of Mouse Anti-IDO1 Antibody. Lane 1: untreated HeLa cells (p/n orb348668). Lane 2: IFN-r treated HeLa cells. Load: 35 µg per lane. Primary antibody: IDO 1 Antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO (p/n orb348624) overnight at 4°C. Predicted/Observed size: 41-42 kDa, 41-42 kDa for IDO-1. Other band(s): none.

Quick Database Links

Gene Symbol

Ido1

UniProt

P14902

RefSeq (Protein):NP_002155.1