iCRT3

SKU: orb1302970

Description

iCRT3 is a small molecule inhibitor that suppresses β-catenin/TCF-dependent transcriptional activity downstream of Wnt signaling. It is utilized in both in vitro and in vivo research to study and target oncogenic Wnt pathway activation in cancer biology and stem cell research.

Research Area

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 901751-47-1
• MW: 394.53
• Purity: 98.47% (May vary between batches)
• Formula: C₂₃H₂₆N₂O₂S
• SMILES: CCc1ccc(cc1)-c1nc(CSCC(=O)NCCc2ccccc2)c(C)o1
• Target: Apoptosis,Wnt/beta-catenin
• Solubility: DMSO:50 mg/mL (126.73 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.07 mM)

Bioactivity

• Target IC50:
  Wnt/β-catenin:8.2 nM
• In Vivo:
  The IL-6 levels in the 10 mg/kg iCRT3 treatment mice are lower.
• In Vitro:
  Treatment with iCRT3 at doses of 12.5, 25, 50, and 75 μM decreases TNF-α levels by 14.7%, 18.5%, 44.9% and 61.3%, respectively.
• Cell Research:
  Cells are seeded into 96-well plates to a density of 5×103 cells per well and incubated in the culture medium with iCRT3 for an additional 48 h. Cell viability and cell apoptosis assays are carried out using a Cell Counting kit-8 and a Caspase-Glo 3/7 assay kit according to the manufacturer's instructions, respectively.
• Animal Research:
  NOD-SCID BALB/c mice inoculated A172 cells are treated with iCRT3 (5 mg/kg) diluted in PBS i.p. triweekly.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

betacatenin, beta-catenin, Apoptosis, bcatenin, inhibit, Inhibitor, iCRT 3, iCRT3, iCRT-3, βcatenin, Wnt/β-catenin, Wnt/betacatenin, Wnt/b-catenin, Wnt

Compound Identifiers

PubChem CID

6622273