Hyocholic acid

SKU: orb2900212

Description

Hyocholic Acid is a bile acid found in pig. Hyocholic Acid can also be found in urine samples from patients with cholestasis. Hyocholic Acid promotes GLP-1 secretion via activating TGR5 and inhibiting FXR in enteroendocrine cells. Hyocholic Acid is known for its exceptional resistance to type 2 diabetes .

Key Properties

• CAS Number: 547-75-1
• MW: 408.57
• Purity: >98% (HPLC)
• Formula: C₂₄H₄₀O₅
• SMILES: [H][C@@]1(CC[C@@]2([H])[C@]3([H])[C@H](O)[C@H](O)[C@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C)[C@H](C)CCC(O)=O
• Solubility: In Vitro: DMSO : 62.5 mg/mL (152.97 mM; Ultrasonic (<60°C)

Bioactivity

• In Vivo:
  Hyocholic Acid (20 mg/kg, p.o.) suppresses BA depletion-induced blood glucose increase in pigs. Hyocholic Acid (100 mg/kg/day, p.o.) improves serum fasting GLP-1 secretion and glucose homeostasis in diabetic mouse models. Animal model: BA depletion pigs. Dosage: 20 mg/kg. Administration: Oral administration (p.o.). Result: Attenuated the increased blood glucose levels corresponding with GLP-1 decrease. Animal model: db/db model, and the high-fat diet and streptozotocin (HFD+STZ) induced diabetic model. Dosage: 100 mg/kg/day. Administration: Oral administration (p.o.). Result: Improved oral glucose tolerances shown by lower glucose levels. Increased circulating active GLP-1 levels and fasting insulin levels.
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• In Vitro:
  HCA (25 and 50 μM, 24 h) upregulates GLP-1 protein secretion in STC-1 and NCI-H716 cells. HCA (25 and 50 μM, 24 h) upregulates proglucagon gene transcription in STC-1 and NCI-H716 cells. Western blot analysis. Cell line: NCI-H716 cells. Concentration: 50 μM. Incubation time: 48 h. Result: Inhibited the high expression of SHP (small heterodimer partner) induced by the FXR agonist.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only