Recombinant Human /Murine/ BDNF Protein

SKU: orb3002487

Description

Recombinant Human Brain-Derived Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding His129-Arg247 is expressed.

Key Properties

• Expression System: E.coli
• Biological Activity: ELISA: Immobilized Human TrkB (C-6His)(Cat. No.:orb2995029) at 5 μg/mL (100 μL/well) can bind Human/Murine/Rat BDNF(Cat. No.:orb3002487)*: Biotinylated by NHS-biotin prior to testing. The EC50 of Human/Murine/Rat BDNF(Cat. No.:orb3002487)* is ≤20 ng/mL.（QC Verified）
• Tag: Tag free
• Molecular Weight: Predicted: 13 KDa. Observed: 14 KDa, reducing conditions
• Expression Region: His129-Arg247
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 0.01 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 400mM NaCl, pH 7.2.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Brain-Derived Neurotrophic Factor; BDNF; Abrineurin

Quick Database Links

UniProt

P23560