Human/mouse/rat MEK1 double mutant protein

SKU: orb420178

Description

Human/mouse/rat MEK1 double mutant protein

Research Area

Signal Transduction

Images & Validation

• Tested Applications: SDS-PAGE, WB
• Application Notes:
  Human MEK1 mutant recombinant protein has been tested in SDS-Page and is suitable as a negative control protein for immunoassays using antibodies targeting the critical MEK1 phosphorylated S218 or S222 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Key Properties

• Expression System: Sf9 cells
• Target: MAP2K1
• Conjugation: Unconjugated
• Purity: MEK1 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. This double mutant protein contain alanine substitutions at S218A and S222A rendering it inactive. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 90% pure.

Storage & Handling

• Storage: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: None
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein

Quick Database Links

Gene Symbol

MAP2K1

UniProt

Q02750