Human/mouse/rat ERK2 double mutant protein

SKU: orb420183

Description

Human/mouse/rat ERK2 double mutant protein

Research Area

Cell Biology

Images & Validation

• Tested Applications: SDS-PAGE, WB
• Application Notes:
  Human ERK1 mutant recombinant protein has been tested in SDS-Page and is suitable as a negative control protein for immunoassays using antibodies targeting the critical ERK1 phosphorylated T202 or Y204 residues. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Key Properties

• Expression System: Sf9 cells
• Target: MAPK3
• Conjugation: Unconjugated
• Purity: ERK1 double mutant human is a recombinant protein containing a polyhistidine tag expressed in Sf9. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK1 and was estimated to be greater than 90% pure.

Storage & Handling

• Storage: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: None
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

MAPK3, ERK, ERK1, PRKM3, ERK-1 recombinant protein

SDS-PAGE results of ERK1 double mutant recombinant Protein. Lane 1: reduced ERK1 DM protein. Lane 2: Opal Prestained Molecular Weight Ladder. Lane 3: non-reduced ERK1 DM protein. Load: 1 µg. 4-20% Lonza SDS-PAGE; Coomassie Stained; BioRad ChemiDoc Imaged.

Quick Database Links

Gene Symbol

MAPK3

UniProt

P27361