Human Y-Box Binding Protein 1 (YBX1) ELISA Kit

SKU: orb778716

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Y-Box Binding Protein 1(YBX1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human YBX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human YBX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human YBX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human YBX1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

BP8 ELISA Kit, CSDA2 ELISA Kit, CSDB ELISA Kit, DBPB ELISA Kit, MDR-NF1 ELISA Kit, NSEP1 ELISA Kit, YB1 ELISA Kit, Nuclease Sensitive Element Binding Protein 1 ELISA Kit, CCAAT-binding transcription factor I subunit A ELISA Kit, DNA-binding protein B ELISA Kit

Quick Database Links

UniProt

P67809