Human WNT1 Inducible Signaling Pathway Protein 3 (WISP3) ELISA Kit

SKU: orb1291324

Description

Human WISP3 (WNT1-inducible-signaling pathway protein 3) ELISA Kit

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 6000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WISP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WISP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WISP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WISP3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 93.75-6000 pg/mL
• Sensitivity: 36.42 pg/mL
• Concentration: 6000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

WISP3, WNT1-inducible-signaling pathway protein 3, CCN6, WISP-3, CCN family member 6

Quick Database Links

UniProt

O95389