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| Catalog Number | orb2667574 |
|---|---|
| Category | Assays and Kits |
| Description | Human WNT Inhibitory Factor 1 (WIF1) ELISA Kit |
| Range | 31.25-2000 pg/mL |
| Assay Time | 3.5h |
| UniProt ID | Q9Y5W5 |
| Sensitivity | 11.4 pg/mL |
| Alternative names | WIF-1 ; wif1 ; WIF1; Wnt inhibitory factor 1 Read more... |
| Note | For research use only |
| Application notes | standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WIF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WIF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WIF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WIF1 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates and other biological fluids |
| Expiration Date | Please enquire. |
- Item 1 of 1
- Item 1 of 1
Human WIF1/Wnt inhibitory factor 1 ELISA Kit [orb381166]
Human
23.4 pg/ml - 1500 pg/ml
< 10 pg/ml
96 T - Item 1 of 1
Human WIF1 ELISA Kit [orb405554]
Human
47 pg/mL-3000 pg/mL
11.7 pg/mL
48 T, 96 T, 10 x 96 T, 5 x 96 T, 24 T
