Human Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit

• Catalog Number: orb1088169
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Von Hippel Lindau Tumor Suppressor(vHL). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Von Hippel Lindau Tumor Suppressor(vHL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Von Hippel Lindau Tumor Suppressor(vHL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Von Hippel Lindau Tumor Suppressor(vHL) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 0.32-20 ng/mL
• Assay Time: 3.5h
• Concentration: 20 ng/mL
• UniProt ID: P40337
• Sensitivity: 0.111 ng/mL
• Alternative names: HRCA1, RCA1, VHL1, Von Hippel-Lindau Syndrome
• Note: For research use only
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human vHL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human vHL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human vHL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human vHL in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Tissue homogenates and other biological fluids.
• Expiration Date: Please enquire.