Human Vitamin D Binding Protein (DBP) ELISA Kit

• Catalog Number: orb2667935
• Category: Assays and Kits
• Description: Human Vitamin D Binding Protein (DBP) ELISA Kit
• UniProt ID: P02774
• Application notes: standard: 100 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DBP in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 1.57-100 µg/mL
• Sensitivity: 0.68 µg/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: GC; VDBG; VDBP; Group-Specific Component; Gc-globu
• Note: For research use only