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Catalog Number | orb257928 |
---|---|
Category | Proteins |
Description | Human VEGF-D, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948). |
Reactivity | Human |
Tag | C-9×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 13.4 kDa |
Target | VEGF-D |
UniProt ID | O43915 |
Source | Human VEGF-D, His Tag (orb257928) is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Human VEGF R3, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
Expression Region | Phe 93 - Ser 201 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH27948 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | FIGF,VEGFD Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 13.4 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human VEGF-D protein
Human VEGF R3, Fc Tag (Cat. No. orb18510) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag (Cat. No. orb257928) with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
> 95% by SDS-PAGE. | |
Recombinant Human VEGF-D Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Phe93-Ser201) of human VEGF-D (Accession #O43915) fused with a 6��His Tag at the C-terminus. |
> 98%, determined by SDS-PAGE | |
This protein contains the mature form of human VEGFD (NP_0044601) corresponding to amino acid (Phe 93-Ser 201) was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells. |
> 95%, determined by SDS-PAGE and SEC-HPLC | |
This protein contains the mature form of human VEGFD (NP_0044601) corresponding to amino acid (Phe 93-Ser 201) was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells. |