Human Vasohibin 1 (VASH1) ELISA Kit

SKU: orb779230

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasohibin 1(VASH1).

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VASH1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VASH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VASH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VASH1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.219 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

Vash1, Vash, Vasohibin-1

Quick Database Links

UniProt

Q7L8A9