Human Vasoactive Intestinal Peptide Receptor 1 (VIPR1) ELISA Kit

SKU: orb776946

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasoactive Intestinal Peptide Receptor 1(VIPR1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VIPR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VIPR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VIPR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VIPR1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HVR1 ELISA Kit, PACAP-R-2 ELISA Kit, RDC1 ELISA Kit, VAPC1 ELISA Kit, VIPR ELISA Kit, VIRG ELISA Kit, VPAC1 ELISA Kit, Pituitary adenylate cyclase-activating polypeptide type II receptor ELISA Kit

Quick Database Links

UniProt

P32241