Human V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1) ELISA Kit

SKU: orb777385

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Set Domain Containing T-Cell Activation Inhibitor 1(VTCN1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VTCN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VTCN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VTCN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VTCN1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

B7-H4 ELISA Kit, B7H4 ELISA Kit, B7S1 ELISA Kit, B7X ELISA Kit, B7h.5 ELISA Kit, VCTN1 ELISA Kit, T-cell costimulatory molecule B7x ELISA Kit, Immune costimulatory protein B7-H4 ELISA Kit

Quick Database Links

UniProt

Q7Z7D3