Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

SKU: orb775718

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Myc Myelocytomatosis Viral Oncogene Homolog(MYC).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYC in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.58 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

C-Myc ELISA Kit, BHLHE39 ELISA Kit, Myc Proto-Oncogene Protein ELISA Kit, Class E basic helix-loop-helix protein 39 ELISA Kit, Transcription factor p64 ELISA Kit, Proto-oncogene c-Myc ELISA Kit