Human V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) ELISA Kit

SKU: orb778565

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3(ErbB3).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ErbB3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ErbB3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ErbB3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ErbB3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 32 pg/mL
• Concentration: 5000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HER3 ELISA Kit, MDA-BF-1 ELISA Kit, C-ErbB3 ELISA Kit, LCCS2 ELISA Kit, ErbB3-S ELISA Kit, P180-ErbB3 ELISA Kit, P45-SErbB3 ELISA Kit, P85-SErbB3 ELISA Kit, Receptor Tyrosine-Protein Kinase ErbB-3 ELISA Kit, Lethal Congenital Contracture Syndrome 2 ELISA Kit