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Human Urotensin 2 (UST2) ELISA Kit
Catalog Number: orb778882
| Catalog Number | orb778882 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Urotensin 2(UST2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Urotensin 2(UST2). |
| Reactivity | Human |
| Concentration | 1000 pg/mL |
| UniProt ID | O95399 |
| Tested applications | ELISA |
| Application notes | standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human UST2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UST2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UST2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 15.63-1000 pg/mL |
| Sensitivity | 4.75 pg/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | UII ELISA Kit, U-II ELISA Kit, UCN2 ELISA Kit, PRO Read more... |
| Research Area | Metabolism Research, Neuroscience |
| Note | For research use only |
| Expiration Date | Please enquire. |
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