Human UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) ELISA Kit

SKU: orb778143

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucuronosyltransferase 1 Family, Polypeptide A1(UGT1A1).

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UGT1A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UGT1A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UGT1A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UGT1A1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.58 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

UGT1 ELISA Kit, GNT1 ELISA Kit, UGT1A ELISA Kit, UDPGT ELISA Kit, HUG-BR1 ELISA Kit, UGT1-1 ELISA Kit, Bilirubin-specific UDPGT isozyme 1 ELISA Kit, UDP-glucuronosyltransferase 1-1 ELISA Kit

Quick Database Links

UniProt

P22309