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| Catalog Number | orb775615 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3A(UBE3A). |
| Reactivity | Human |
| Concentration | 20 ng/mL |
| UniProt ID | Q05086 |
| Tested applications | ELISA |
| Application notes | standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UBE3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UBE3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UBE3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UBE3A in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.122 ng/mL |
| Sample Types | tissue homogenates, cell lysates and other biological fluids |
| Alternative names | ANCR ELISA Kit, AS ELISA Kit, E6-AP ELISA Kit, EPV Read more... |
| Note | For research use only |
- Item 1 of 1
- Item 1 of 1
Human Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit [orb1949096]
Human
0.32-20ng/mL
0.19ng/mL
48 T, 96 T 
