Human Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ELISA Kit

• Catalog Number: orb781347
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1(UCHL1).
• Reactivity: Human
• Concentration: 50 ng/mL
• UniProt ID: P09936
• Tested applications: ELISA
• Application notes: standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UCHL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UCHL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UCHL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UCHL1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.79-50 ng/mL
• Sensitivity: 0.28 ng/mL
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: PARK5 ELISA Kit, PGP9.5 ELISA Kit, Uch-L1 ELISA Ki
• Research Area: Protein Biochemistry
• Note: For research use only
• Expiration Date: Please enquire.

References

Włodzimierz Płotek 1, Artur Bekała 2, Jadwiga Łuczak-Wawrzyniak 2, Katarzyna Dudzińska-Rapczewska 2, Małgorzata Gasińska-Błotniak 1, Marcin Cybulski 3, Agnieszka Kubik-Komar 4, Elżbieta Kubera 4, Maciej Wilczak 5, Wojciech Dąbrowski 6 Is general anesthesia neuroinjury-free and safe for gynecological patients' cognition? A comparison of two typical anesthesia schemes based on propofol and sevoflurane Anaesthesiol Intensive Ther, (2025)

PubMed: 40891792

Applications

ELISA

Reactivity

Human