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Human UBE2T protein

SKU: orb392669

Description

Recombinant human UBE2T protein is produced by our E. coli expression system and the target gene encoding Met1-Val197 is expressed with a 6His tag at the N-terminus.

Research Area

Epigenetics & Chromatin

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetUBE2T
ReactivityHuman
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Protein SequenceMGSSHHHHHH SSGLVPRGSH MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDV

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Ubiquitin-conjugating enzyme E2 T; Cell proliferation-inducing gene 50 protein; Ubiquitin carrier protein T; Ubiquitin-protein ligase T

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human UBE2T protein (orb392669)

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Available Sizes

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10 μg
$ 110.00
50 μg
$ 240.00
500 μg
$ 1,610.00
DispatchUsually dispatched within 5-10 working days
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