Human Tyrosine-Protein Phosphatase Non-Receptor Type 5 (PTPN5) ELISA Kit

SKU: orb1146771

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine-Protein Phosphatase Non-Receptor Type 5(PTPN5). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tyrosine-Protein Phosphatase Non-Receptor Type 5(PTPN5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine-Protein Phosphatase Non-Receptor Type 5(PTPN5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosine-Protein Phosphatase Non-Receptor Type 5(PTPN5) in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTPN5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTPN5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTPN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTPN5 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.052 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

FLJ14427, Neural specific protein tyrosine phosphatase, Neural-specific protein-tyrosine phosphatase, Protein tyrosine phosphatase non receptor type 5 (striatum enriched), Protein tyrosine phosphatase non receptor type 5, Protein tyrosine phosphatase striatum enriched, PTN5, PTN5_HUMAN, PTP STEP, PTPN 5, Ptpn5, PTPSTEP, STEP, Striatum-enriched protein-tyrosine phosphatase, Tyrosine protein phosphatase non receptor type 5, Tyrosine-protein phosphatase non-receptor type 5,

Quick Database Links

UniProt

P54829