Human Tyrosine-protein kinase ABL1 (ABL1) ELISA Kit

SKU: orb1291341

Description

Human ABL1 (Tyrosine-protein kinase ABL1) ELISA Kit

Research Area

Cancer Biology, Epigenetics & Chromatin, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ABL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ABL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ABL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ABL1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 62.5-4000 pg/mL
• Sensitivity: 17 pg/mL
• Concentration: 4000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

ABL1, ABL, JTK7, Tyrosine-protein kinase ABL1, Abelson murine leukemia viral oncogene homolog 1, Abelson tyrosine-protein kinase 1, Proto-oncogene c-Abl, p150

Quick Database Links

UniProt

P00519