Human Tumor Protein, Translationally Controlled 1 (TPT1) ELISA Kit

SKU: orb777875

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Protein, Translationally Controlled 1(TPT1).

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPT1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.12 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HRF ELISA Kit, TCTP ELISA Kit, p02 ELISA Kit, p23 ELISA Kit, Fortilin ELISA Kit, Translationally-Controlled Tumor Protein ELISA Kit, Histamine-releasing factor ELISA Kit

Quick Database Links

UniProt

P13693