Human Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) ELISA Kit

SKU: orb778342

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 14(TNFSF14).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFSF14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFSF14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFSF14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNFSF14 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.4 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD258 ELISA Kit, TR2 ELISA Kit, HVEML ELISA Kit, LIGHT ELISA Kit, LTg ELISA Kit, Herpes virus entry mediator ligand ELISA Kit, Herpesvirus entry mediator ligand ELISA Kit

Quick Database Links

UniProt

O43557