Human Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12) ELISA Kit

• Catalog Number: orb778263
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 12(TNFSF12).
• Tested applications: ELISA
• Reactivity: Human
• Range: 15.63-1000 pg/mL
• Assay Time: 3.5h
• Concentration: 1000 pg/mL
• UniProt ID: O43508
• Sensitivity: 6.4 pg/mL
• Alternative names: APO3L ELISA Kit, DR3LG ELISA Kit, TWEAK ELISA Kit,
• Note: For research use only
• Application notes: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFSF12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFSF12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFSF12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNFSF12 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Expiration Date: Please enquire.