Human Trefoil Factor 1 (TFF1) ELISA Kit

SKU: orb775610

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trefoil Factor 1(TFF1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TFF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TFF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TFF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TFF1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.056 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

BCEI ELISA Kit, HP1.A ELISA Kit, HPS2 ELISA Kit, PS2 ELISA Kit, PNR2 ELISA Kit, Breast Cancer,Estrogen-Inducible Sequence Expressed In ELISA Kit, Breast cancer estrogen-inducible protein ELISA Kit, Polypeptide P1.A ELISA Kit

Quick Database Links

UniProt

P04155