Human Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit

SKU: orb776655

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta Induced Protein(TGFbI).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TGFbI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TGFbI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TGFbI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TGFbI in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.232 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

TGF-BI ELISA Kit, CSD3 ELISA Kit, LCD1 ELISA Kit, CSD1 ELISA Kit, CSD2 ELISA Kit, BIGH3 ELISA Kit, CDB1 ELISA Kit, CDGG1 ELISA Kit, RGD-CAP ELISA Kit, Kerato-Epithelin ELISA Kit, RGD-Containing Collagen-Associated Protein ELISA Kit

Quick Database Links

UniProt

Q15582