Human Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22) ELISA Kit

SKU: orb775995

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta Stimulated Protein Clone 22(TSC22).

Research Area

Cancer Biology, Infectious Disease & Virology, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSC22. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSC22. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSC22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSC22 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.108 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

TSC22D1 ELISA Kit, TGFB1I4 ELISA Kit, TSC22 ELISA Kit, hucep-2 ELISA Kit, Cerebral protein 2 ELISA Kit, Transforming Growth Factor Beta 1 Induced Transcript 4 ELISA Kit, TSC22 Domain Family,Member 1 ELISA Kit