Human Transferrin protein (TRITC)

SKU: orb346214

Description

Human Transferrin protein conjugated to TRITC

Images & Validation

• Tested Applications: DOT
• Application Notes:
  Human transferrin rhodamine conjugation has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Source: Human
• Biological Origin: Human
• Isotype: Transferrin
• Purity: This product was prepared from normal serum by delipidation, salt fractionation, selective precipitation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Transferrin and anti-Human Serum.
• Conjugation: TRITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/ml
• Disclaimer: For research use only

Alternative Names

Human transferrin Rhodamine conjugation, TRITC conjugated transferrin

Colocalization of ATP7B with TGN46 or LAMP1. Cells were left untreated (basal medium), treated with 10 µm TTM (low copper) or treated with 10, 100 or 200 µm CuCl2. (A, B) Merged images show ATP7B in green, TGN46 (A) or LAMP1 (B) in magenta and the nucleus in blue; pixel overlap is shown in white.

COMMD1–PtdIns(4, 5)P2 interaction measured by fluorescence quenching of all aromatics. (C) Example images showing colocalization of ATP7B with wild type, T174M and K167/173E COMMD1. ATP7B is in green and COMMD1 variants in magenta as indicated.

Dot Blot of Rhodamine Conjugated Human Transferrin. Dotted directly with Rhodamine Conjugated Human Transferrin at following concentrations. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng Primary antibody: none Secondary antibody: none Block: orb348637 for 1 HR at RT.

Rab5c function is in an EC autonomous manner. (A) TRITC-conjugated TF internalization assay in Hela cells transfected with empty pCS2 or pCS2-rab5c DN plasmids. Representative pictures were shown. Scale bar, 10 µm. (B) Quantitative fluorescence intensity of intracellular TRITC-TF in empty pCS2 or pCS2-rab5c DN transfected Hela cells, n = 8 cells for each group. Error bars, mean ± SD. P value was calculated by Student t test, ***P < 0.001. DN, dominant-negative; TF, transferrin; TRITC, tetramethylrhodamine.

Rab5c over activation leads to excessive endolysosomal trafficking of Notch ligands and receptor. (A) TRITC-conjugated TF internalization assay in Hela cells transfected with control empty pCS2 or pCS2-rab5c CA plasmid. Representative pictures were shown. Scale bar, 10 µm. (B) Quantitative fluorescence intensity of intracellular TRITC-TF in control empty pCS2 and pCS2-rab5c CA transfected cells, n = 8 cells for each group. Error bars, mean ± SD. P value was calculated by Student t test, ***P < 0.001. CA, constitutively active; TF, transferrin; TRITC, tetramethylrhodamine.

Quick Database Links

UniProt

Q06AH7

RefSeq:AAB22049.1