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Catalog Number | orb706862 |
---|---|
Category | Proteins |
Description | Recombinant Human CD258 Protein is produced by mammalian expression system and the target gene encoding Leu83-Val240 is expressed with a 6His tag at the N-terminus. |
Target | TNFSF14 |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Protein Sequence | HHHHHHGLIQ ERRSHEVNPA AHLTGANSSL TGSGGPLLWE TQLGLAFLRG LSYHDGALVV TKAGYYYIYS KVQLGGVGCP LGLASTITHG LYKRTPRYPE ELELLVSQQS PCGRATSSSR VWWDSSFLGG VVHLEAGEEV VVRVLDERLV RLRDGTRSYF GAFMV |
UniProt ID | O43557 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | HVEML protein, LIGHT protein, Tumor necrosis facto Read more... |
Note | For research use only |
Entrez | 8740 |
Expiration Date | 6 months from date of receipt. |
Greater than 85% as determined by SDS-PAGE. | |
46.7 kDa | |
Mammalian cell |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 43.4 kDa after removal of the signal peptide. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 52-140 kDa based on Tris-Bis PAGE result. |
> 95% as determined by Tris-Bis PAGE | |
Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. |
Greater than 90% as determined by SDS-PAGE. | |
48.3 kDa | |
Mammalian cell |