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Human TNFSF14 protein
Description
Research Area
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Source | Mammalian cell |
|---|---|
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized TNFRSF14 at 5 μg/ml can bind TNFSF14, the EC50 is 45.44-53.29 ng/ml. |
| Tag | N-terminal hFC-Myc-tagged |
| Molecular Weight | 46.7 kDa |
| Expression Region | 74-240aa |
| Protein Length | Partial |
| Protein Sequence | DGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSSSRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Endotoxins | Less than 1.0 EU/μg as determined by LAL method. |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
|---|---|
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4 |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human LIGHT Protein, hFc Tag [orb757459]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 43.4 kDa after removal of the signal peptide.
Mammalian
50 μg, 100 μg, 10 μgHuman TNFSF14 protein [orb605256]
Greater than 90% as determined by SDS-PAGE.
48.3 kDa
Mammalian cell
20 μg, 100 μg, 1 mgLIGHT (TNFSF14) (NM_003807) Human Recombinant Protein [orb3045750]
> 80% as determined by SDS-PAGE and Coomassie blue staining
22 kDa
20 μg, 100 μg, 1 mgRecombinant Human CD258/TNFSF14/LIGHT Protein, N-His [orb2969914]
>90% as determined by SDS-PAGE.
20.52 kDa
1 mg, 100 μg, 50 μg

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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized TNFRSF14 at 5 μg/ml can bind TNFSF14, the EC50 is 45.44-53.29 ng/ml.
Quick Database Links
UniProt Details
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Protocol Information
Human TNFSF14 protein (orb705344)
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