Human TNFRSF1B Protein, hFc Tag

• Catalog Number: orb1743387
• Category: Proteins
• Description: Recombinant Human TNFRSF1B Protein with C-terminal human Fc tag
• Target: TNFRSF1B
• Tag: C-Human Fc Tag
• Reactivity: Human
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P20333
• MW: The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide. The apparent molecular mass of TNFRSF1B-hFc is approximately 55-70kDa due to glycosylation.
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Alternative names: CD120b;p75;p75TNFR;TBPII;TNF-R-II;TNF-R75;TNFBR;TN
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human B7-2 Protein, hFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-2, hFc tagged protein (orb689458) can bind Human CTLA4, mFc-His tagged protein orb689378 in a linear range of 0.048-2.694 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-2, hFc tagged protein (orb689458) can bind Human CD28, mFc-His tagged protein orb689375 in a linear range of 62.5-4000 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human CD28 (A) were surface stained with Human B7-2, hFc tagged protein (orb689458) 1 μg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.