Human TNF Receptor Associated Factor 3 (TRAF3) ELISA Kit

SKU: orb2667729

Description

Human TNF Receptor Associated Factor 3 (TRAF3) ELISA Kit

Research Area

Cancer Biology

Images & Validation

• Application Notes:
  standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRAF3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRAF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRAF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRAF3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.78-50 ng/mL
• Sensitivity: 0.18 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

LAP1; CAP-1; CD40bp; CRAF1; CD40 receptor-associated factor 1; CD40-binding protein; LMP1-associated protein 1

Quick Database Links

UniProt

Q13114